2OML
crystal structure of E. coli pseudouridine synthase RluE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-02 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.310, 56.780, 91.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.080 - 1.200 |
R-factor | 0.152 |
Rwork | 0.149 |
R-free | 0.17900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1KSV with N-terminal domain removed |
RMSD bond length | 0.014 |
RMSD bond angle | 1.672 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.240 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.063 | 0.713 |
Number of reflections | 52181 | |
<I/σ(I)> | 13.7 | 2.1 |
Completeness [%] | 98.1 | 94 |
Redundancy | 4.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 294 | 4 mg/ml protein in 10 mM Tris, pH 7.5, 2mM EDTA and 2mM DTT equilibrated against 22% (w/v) MME PEG 2000, 200 mM ammonium sulfate, 100 mM sodium acetate, crystals were improved by microseeding a solution of 18-22% MME PEG 2000, 200 mM ammonium sulfate, 100 mM sodium acetate and 2 mg/ml protein at pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |