2OME
Crystal structure of human CTBP2 dehydrogenase complexed with NAD(H)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.99991 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.754, 141.574, 138.215 |
Unit cell angles | 90.00, 98.93, 90.00 |
Refinement procedure
Resolution | 43.310 - 2.800 |
R-factor | 0.2098 |
Rwork | 0.207 |
R-free | 0.25736 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 1MX3 1hl3 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.458 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.310 | 2.920 |
High resolution limit [Å] | 2.770 | 2.770 |
Rmerge | 0.148 | 0.810 |
Number of reflections | 84796 | |
<I/σ(I)> | 11.6 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.1 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2M KSCN, 0.1M BIS-TRIS PROPANE, 20% PEG3350, 10% ETHYLENE GLYCOL, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |