2OME
Crystal structure of human CTBP2 dehydrogenase complexed with NAD(H)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99991 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.754, 141.574, 138.215 |
| Unit cell angles | 90.00, 98.93, 90.00 |
Refinement procedure
| Resolution | 43.310 - 2.800 |
| R-factor | 0.2098 |
| Rwork | 0.207 |
| R-free | 0.25736 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1MX3 1hl3 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.458 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.310 | 2.920 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.148 | 0.810 |
| Number of reflections | 84796 | |
| <I/σ(I)> | 11.6 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2M KSCN, 0.1M BIS-TRIS PROPANE, 20% PEG3350, 10% ETHYLENE GLYCOL, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
