2OMB
Bence Jones KWR Protein- Immunoglobulin Light Chain Dimer, P3(1)21 Crystal Form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-01-14 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.080 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 153.520, 153.520, 93.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.900 |
R-factor | 0.238 |
Rwork | 0.235 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dcl |
RMSD bond length | 0.008 |
RMSD bond angle | 26.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 3.020 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 27674 | |
<I/σ(I)> | 14.7 | 2.3 |
Completeness [%] | 96.6 | 90.1 |
Redundancy | 4.6 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 1.5 M Ammonium Sulfate, 0.1M Tris/HCl pH8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |