2OM6
Hypothetical Protein (Probable Phosphoserine Phosph (PH0253) from Pyrococcus Horikoshii OT3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-07 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.9792, 0.9000, 0.9797 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.052, 66.239, 79.014 |
| Unit cell angles | 90.00, 107.62, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.25900 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.079 | 0.204 |
| Number of reflections | 24370 | |
| Completeness [%] | 98.0 | 91.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 293 | 0.1M Tris-HCl, 50% Glycerol, 0.2M Amm.Sulfate, 17.5% PEG 3350, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
