2OLV
Structural Insight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Donor Ligand Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 79.565, 212.211, 91.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.150 - 2.800 |
| R-factor | 0.234 |
| Rwork | 0.231 |
| R-free | 0.28000 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.332 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.151 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.083 | 0.565 |
| Total number of observations | 27580 | |
| Number of reflections | 39086 | |
| <I/σ(I)> | 6.8 | 1.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 100mM Na citrate, 0.1M NaCl, 0.1M MgCl2, 12% PEG 4000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
