2OLU
Structural Insight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Apoenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 70.495, 249.891, 103.164 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.600 - 2.900 |
R-factor | 0.194 |
Rwork | 0.191 |
R-free | 0.24600 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.507 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.600 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.132 | 0.431 |
Total number of observations | 12734 | |
Number of reflections | 20671 | |
<I/σ(I)> | 4.9 | 1.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.2 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 100mM hepes, 0.2M KCl, 4% PEG 8000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |