2OLT

Crystal structure of a phou-like protein (so_3770) from shewanella oneidensis mr-1 at 2.00 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	H 3 2
Unit cell lengths	99.530, 99.530, 395.920
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.160 - 2.000
R-factor	0.205
Rwork	0.203
R-free	0.24200
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.565
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.160	50.000	2.050
High resolution limit [Å]	2.000	4.820	2.000
Rmerge	0.058	0.029	0.623
Number of reflections	51605
<I/σ(I)>	13.4
Completeness [%]	99.9	98.4	100
Redundancy	5.5	5.2	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	277	NANODROP, 1.5M Sodium acetate, 0.1M Sodium cacodylate pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K
2	VAPOR DIFFUSION, SITTING DROP	6.5	277	NANODROP, 1.0M Sodium citrate, 0.1M Sodium cacodylate pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K