2OLQ
How Does an Enzyme Recognize CO2?
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-17 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.584, 91.369, 46.577 |
| Unit cell angles | 90.00, 96.22, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.940 |
| R-factor | 0.204 |
| Rwork | 0.205 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ayl |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.040 |
| High resolution limit [Å] | 1.720 | 1.940 |
| Rmerge | 0.066 | 0.516 |
| Number of reflections | 52927 | |
| <I/σ(I)> | 7.4 | 1.5 |
| Completeness [%] | 95.6 | 76.1 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 298 | 30% P4000, 0.1 M sodium acetate pH 4.5, 0.2 M ammonium acetate, VAPOR DIFFUSION, temperature 298K |
