2OLM

ArfGap domain of HIV-1 Rev binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-19
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00000
Spacegroup name	P 43 21 2
Unit cell lengths	57.883, 57.883, 83.696
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.480
Rwork	0.180
R-free	0.19480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2iqj
RMSD bond length	0.016
RMSD bond angle	1.459
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.480	3.190	1.480
Rmerge	0.055	0.042	0.237
Number of reflections	45446	4570	4449
<I/σ(I)>	18.9
Completeness [%]	99.7	99.5	97.3
Redundancy	7.2	7.5	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	2.27M Ammonium phosphate, 0.09M BTP, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K