2OLK
ABC Protein ArtP in complex with ADP-beta-S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.290, 54.117, 106.006 |
| Unit cell angles | 90.00, 90.15, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| Rwork | 0.204 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Complex of ArtP with SO4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.344 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.098 | 0.381 |
| Number of reflections | 54247 | |
| <I/σ(I)> | 6.2 | 1.66 |
| Completeness [%] | 92.8 | 68.7 |
| Redundancy | 3.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 30% PEG 400, 0.1M natrium acetate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
