2OL8
The crystal structure of OspA mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97934 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.356, 53.226, 65.195 |
Unit cell angles | 90.00, 98.83, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.23443 |
Rwork | 0.232 |
R-free | 0.28612 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g8c |
RMSD bond length | 0.018 |
RMSD bond angle | 1.670 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.064 | 0.335 |
Number of reflections | 20849 | |
<I/σ(I)> | 27.43 | 4.17 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 4 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.45 | 293 | 38% PEG400, and 100mM Imidazole, pH 6.45, VAPOR DIFFUSION, HANGING DROP, temperature 293K |