2OL7
The crystal structure of OspA mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-21 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 37.127, 55.333, 64.789 |
Unit cell angles | 85.68, 74.05, 86.45 |
Refinement procedure
Resolution | 20.000 - 1.350 |
R-factor | 0.1854 |
Rwork | 0.184 |
R-free | 0.21819 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g8c |
RMSD bond length | 0.013 |
RMSD bond angle | 1.479 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.400 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.069 | 0.324 |
Number of reflections | 103051 | |
<I/σ(I)> | 18.87 | 4.34 |
Completeness [%] | 95.6 | 93.1 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG3350, 6% isopropanol, 200mM NaCl, and 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |