2OL6
The crystal structure of OspA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.882, 51.508, 65.639 |
| Unit cell angles | 90.00, 98.56, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.22005 |
| Rwork | 0.218 |
| R-free | 0.25877 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g8c |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.569 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.048 | 0.475 |
| Number of reflections | 28159 | |
| <I/σ(I)> | 21.5 | 2.96 |
| Completeness [%] | 97.0 | 95.6 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | 32% PEG400, 4% MPD, and 100mM Imidazole, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
