2OL5
Crystal Structure of a protease synthase and sporulation negative regulatory protein PAI 2 from Bacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-20 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.601, 77.949, 89.453 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.810 - 2.500 |
| R-factor | 0.21911 |
| Rwork | 0.213 |
| R-free | 0.27551 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.764 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.067 | 0.341 |
| Number of reflections | 34879 | |
| <I/σ(I)> | 8.7 | 2.27 |
| Completeness [%] | 81.7 | 36.7 |
| Redundancy | 4.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.1M Ammonium acetate, 0.1M Bis-Tris pH 6.0, 17% PEG 10000, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
