2OKU
The crystal structure of the acyl-CoA dehydrogenase family protein from Porphyromonas gingivalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97980 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 90.340, 90.340, 102.069 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.570 - 1.900 |
| R-factor | 0.17498 |
| Rwork | 0.173 |
| R-free | 0.20541 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.342 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.570 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.114 | 0.800 |
| Number of reflections | 32154 | |
| <I/σ(I)> | 31.53 | 2.27 |
| Completeness [%] | 99.9 | 99.15 |
| Redundancy | 18.9 | 17.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 1.26M (NH4)2SO4, 0.1M Tris-HCl, 0.2M LiSO4, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
