2OKG
Structure of effector binding domain of central glycolytic gene regulator (CggR) from B. subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97900 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.603, 83.804, 116.721 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.040 - 1.650 |
R-factor | 0.19792 |
Rwork | 0.196 |
R-free | 0.23871 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.479 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.087 | 0.627 |
Number of reflections | 62805 | |
<I/σ(I)> | 52.2 | 3.6 |
Completeness [%] | 97.1 | 99.2 |
Redundancy | 5.3 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Reservoir: 16% PEG3350, 0.1M MES pH 6.5, 0.1M MgCl2, 0.1M 6-aminohexanoic acid. Protein: 26.2mg/ml. Drops: 1+1 microliter, VAPOR DIFFUSION, HANGING DROP, temperature 298K |