2OKB
High Resolution Crystal Structures of Vaccinia Virus dUTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-05 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 63 |
| Unit cell lengths | 105.260, 105.260, 59.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.130 - 2.150 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q5u |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.902 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0011) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.700 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.033 | 0.144 |
| Number of reflections | 20238 | |
| <I/σ(I)> | 29.9 | 6 |
| Completeness [%] | 98.6 | 92.1 |
| Redundancy | 6.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | 298 | 12% PEG1000, 0.1M lithium sulfate, 0.1M phosphate/citrate buffer, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 4.20 |
