2OJE
Mycoplasma arthritidis-derived mitogen complexed with class II MHC molecule HLA-DR1/HA complex in the presence of EDTA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 137.735, 179.555, 179.952 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.220 - 3.000 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.26100 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1r5i |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.220 | 3.190 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.124 | 0.563 |
Number of reflections | 40345 | |
<I/σ(I)> | 17 | 3.2 |
Completeness [%] | 89.8 | 91.5 |
Redundancy | 5.2 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | 1.7M potassium sodium phosphate, 0.1M HEPES, 2 mM EDTA, pH 7.5, VAPOR DIFFUSION, temperature 298K |