2OJ6
Crystal Structure of Reovirus T3D Attachment Protein Sigma1 head domain D345N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-19 |
| Detector | MAR CCD 225 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.020, 51.600, 108.860 |
| Unit cell angles | 90.00, 95.59, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| R-factor | 0.1801 |
| Rwork | 0.175 |
| R-free | 0.22364 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | residues 293-455 of 1KKE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.202 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.096 | 0.416 |
| Number of reflections | 78687 | |
| <I/σ(I)> | 6.5 | 1.4 |
| Completeness [%] | 98.7 | 97.9 |
| Redundancy | 4.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293 | 0.1 M sodium cacodylate, 0.2 M magnesium sulfate, 20% PEG 8000; protein concentration 8 mg/ml, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
