2OJ4
Crystal structure of RGS3 RGS domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-14 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.236, 60.849, 57.406 |
| Unit cell angles | 90.00, 135.44, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.221 |
| Rwork | 0.193 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fqi |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.890 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.055 | 0.214 |
| Number of reflections | 8095 | |
| <I/σ(I)> | 16.5 | 5.3 |
| Completeness [%] | 96.7 | 97.3 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 28% PEG 4000, 0.15M magnesium chloride, 0.1M glycine, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
