2OJ1
Disulfide-linked dimer of azurin N42C/M64E double mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-24 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 68.570, 116.980, 30.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.300 - 2.300 |
| Rwork | 0.208 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | P. aeruginosa azurin wildtype |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.843 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.300 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.095 | 0.490 |
| Number of reflections | 10369 | |
| <I/σ(I)> | 8.3 | 2.4 |
| Completeness [%] | 95.1 | 95.6 |
| Redundancy | 3.88 | 2.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.1 | 293 | 30 % PEG 4000, 0.1M sodium citrate buffer, pH 3.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
