2OJ0
Crystal structure of the duplex form of the HIV-1(LAI) RNA dimerization initiation site MN soaked
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 42.538, 47.834, 58.681 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.970 - 2.600 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.23000 |
| Structure solution method | MIR |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 7223 | |
| <I/σ(I)> | 21.1 | 12 |
| Completeness [%] | 98.4 | 92.7 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | MPD, POTASSIUM CHLORIDE, MAGNESIUM CHLORIDE, CACODYLATE, SPERMINE, pH 7.00, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 2 | CACODYLATE | ||
| 11 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | POTASSIUM CHLORIDE | ||
| 3 | 1 | 1 | MAGNESIUM CHLORIDE | ||
| 4 | 1 | 1 | CACODYLATE | ||
| 5 | 1 | 1 | SPERMINE | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | MPD | ||
| 8 | 1 | 2 | POTASSIUM CHLORIDE | ||
| 9 | 1 | 2 | MAGNESIUM CHLORIDE |
