2OIU
L1 Ribozyme Ligase circular adduct
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9840 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.290, 100.018, 71.930 |
Unit cell angles | 90.00, 104.42, 90.00 |
Refinement procedure
Resolution | 31.340 - 2.600 |
R-factor | 0.2065 |
Rwork | 0.203 |
R-free | 0.23776 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.976 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0012) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.044 | 0.467 |
Number of reflections | 19071 | |
<I/σ(I)> | 20.2 | 2.5 |
Completeness [%] | 99.5 | 99.9 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 1.8M lithium sulfate, 10mM magnesium sulfate, 50mM sodium cacodylate (pH 6.0), 100mM sodium chloride, 60mM magnesium chloride, 30mM tris (pH 7.6), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | lithium sulfate | ||
10 | 1 | 2 | sodium chloride | ||
11 | 1 | 2 | magnesium chloride | ||
12 | 1 | 2 | tris | ||
2 | 1 | 1 | magnesium sulfate | ||
3 | 1 | 1 | sodium cacodylate | ||
4 | 1 | 1 | sodium chloride | ||
5 | 1 | 1 | magnesium chloride | ||
6 | 1 | 1 | tris | ||
7 | 1 | 2 | lithium sulfate | ||
8 | 1 | 2 | magnesium sulfate | ||
9 | 1 | 2 | sodium cacodylate |