2OIT

Crystal Structure of the N-terminal Domain of the Human Proto-oncogene Nup214/CAN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-19
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	52.381, 81.140, 102.607
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.650
Rwork	0.199
R-free	0.23700
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.710
High resolution limit [Å]	1.650	3.550	1.650
Rmerge	0.064	0.031	0.840
Number of reflections	49618
<I/σ(I)>	14.2
Completeness [%]	99.3	99.5	93.9
Redundancy	20.3	14	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	100 mM MES pH 6. 23-27 % PEG 2,000 MME, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	100 mM MES pH 6. 23-27 % PEG 2,000 MME, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K