2OIK
Crystal structure of a histidine triad (hit) protein (mfla_2506) from methylobacillus flagellatus kt at 1.65 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-17 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.243, 47.981, 69.766 |
| Unit cell angles | 108.13, 98.22, 90.34 |
Refinement procedure
| Resolution | 45.549 - 1.650 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.22300 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.565 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.549 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.118 | 0.828 |
| Number of reflections | 62091 | |
| <I/σ(I)> | 7.36 | 1.52 |
| Completeness [%] | 93.5 | 70.5 |
| Redundancy | 3.94 | 3.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 277 | NANODROP, 0.1M NaCl, 30.0% PEG MME-550, 0.1M Bicine pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 9.00 |
