2OI9
Structure of the 2C/Ld/QL9 allogeneic complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97908 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 163.171, 163.171, 95.028 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.000 - 2.350 |
| R-factor | 0.22076 |
| Rwork | 0.220 |
| R-free | 0.22629 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ldp 2ckb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.062 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 141.420 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.078 | 0.488 |
| Number of reflections | 29820 | |
| <I/σ(I)> | 17.3 | 2.5 |
| Completeness [%] | 99.9 | 99.96 |
| Redundancy | 5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 298 | 0.9M sodium dihydrogen phosphate, 0.01M di-potassium hydrogen phosphate, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
