2OHV
Structural Basis for Glutamate Racemase Inhibition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-16 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 79.745, 79.745, 95.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.870 - 2.500 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ohg |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.074 | 0.289 |
Number of reflections | 12607 | |
<I/σ(I)> | 42 | 4.1 |
Completeness [%] | 99.3 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 295 | 8% PEG6000, 7% Glycerol, 0.1M Bicine, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |