2OHI
Crystal Structure of coenzyme F420H2 oxidase (FprA), a diiron flavoprotein, reduced state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Detector technology | CCD |
Collection date | 2006-04-27 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 97.798, 123.113, 135.864 |
Unit cell angles | 90.00, 103.40, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.2061 |
Rwork | 0.203 |
R-free | 0.26907 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.363 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | EPMR |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 103861 | |
<I/σ(I)> | 13.7 | 1.8 |
Completeness [%] | 97.6 | 97.8 |
Redundancy | 2.6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 283 | 0.001M DDT, 0.2M ammonium sulfate, 0.1M MES/KOH, 30% PEG MME 5000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |