2OHD
Crystal structure of hypothetical molybdenum cofactor biosynthesis protein C from Sulfolobus tokodaii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.310, 78.578, 112.671 |
| Unit cell angles | 90.00, 118.06, 90.00 |
Refinement procedure
| Resolution | 45.780 - 2.200 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ekr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.055 | 0.268 |
| Number of reflections | 48362 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 18% PEG 3000, 0.1M HEPES, 0.2M magnesium chloride , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
