2OHB
Myoglobin cavity mutant I107W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-10 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 6 |
| Unit cell lengths | 91.560, 91.560, 46.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 79.290 - 1.800 |
| Rwork | 0.177 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 79.290 | 100.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 4.120 | 1.800 |
| Rmerge | 0.075 | 0.037 | 0.355 |
| Number of reflections | 18360 | ||
| <I/σ(I)> | 13 | ||
| Completeness [%] | 88.4 | 98.7 | 23.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | PROTEIN SOLUTION (20 MG/ML PROTEIN, 0.020 M TRIS-HCL, pH 9.0), MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (2.8 M AMMONIUM SULPHATE, 0.050 M TRIS-HCL, pH 9.0), vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP |
