2OHA
Myoglobin cavity mutant F138W
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-03-15 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 6 |
Unit cell lengths | 90.180, 90.180, 45.340 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.590 - 1.800 |
Rwork | 0.166 |
R-free | 0.20000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.800 |
Data scaling software | d*TREK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.590 | 29.590 | 1.590 |
High resolution limit [Å] | 1.540 | 3.310 | 1.540 |
Rmerge | 0.048 | 0.037 | 0.144 |
Total number of observations | 10996 | 649 | |
Number of reflections | 26942 | ||
<I/σ(I)> | 16 | 31.4 | 2.6 |
Completeness [%] | 85.4 | 99.7 | 19.4 |
Redundancy | 2.89 | 3.32 | 1.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | PROTEIN SOLUTION (20 MG/ML PROTEIN, 0.020 M TRIS-HCL, pH 9.0), MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (2.8 M AMMONIUM SULPHATE, 0.050 M TRIS-HCL, pH 9.0), cryo-protected with 20% SUCROSE, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP |