2OH9

Myoglobin cavity mutant V68W

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	277
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 6
Unit cell lengths	91.490, 91.490, 45.890
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.890 - 1.800
Rwork	0.174
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.600
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.890
High resolution limit [Å]	1.700
Number of reflections	24077

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	PROTEIN SOLUTION (20 MG/ML PROTEIN, 0.020 M TRIS-HCL, pH 9.0), MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (2.8 M AMMONIUM SULPHATE, 0.050 M TRIS-HCL, pH9.0), vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP