2OH1
Crystal structure of acetyltransferase GNAT family (YP_013287.1) from Listeria monocytogenes 4b F2365 at 1.46 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-03 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97932, 0.97901 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.624, 99.833, 122.878 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.225 - 1.460 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.19300 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.631 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.225 | 1.510 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.055 | 0.671 |
| Number of reflections | 125870 | |
| <I/σ(I)> | 12.54 | 1.79 |
| Completeness [%] | 89.6 | 52.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 277 | NANODROP, 1.0M Na Citrate, 0.2M NaCl, 0.1M TRIS pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 7.00 |
