2OGU
Crystal structure of the isolated MthK RCK domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97944 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 165.890, 165.890, 82.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.230 |
| R-factor | 0.23467 |
| Rwork | 0.232 |
| R-free | 0.29937 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lmq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.355 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.068 | 0.293 |
| Number of reflections | 6717 | |
| <I/σ(I)> | 21.5 | 5.2 |
| Completeness [%] | 91.7 | 59.7 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1M Sodium cacodylate pH 6.5, 1.3-1.5M Lithium sulfate, 2% Benzamidine-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
