2OGQ
Molecular and structural basis of Plk1 substrate recognition: Implications in centrosomal localization
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-05 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 77.051, 99.017, 33.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.860 - 1.950 |
R-factor | 0.16219 |
Rwork | 0.159 |
R-free | 0.22125 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1umw |
RMSD bond length | 0.025 |
RMSD bond angle | 1.951 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.000 | 2.050 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 16920 | |
<I/σ(I)> | 1.3 | |
Completeness [%] | 93.9 | 99.9 |
Redundancy | 3.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 9 | 289 | 0.1 M Sodium Chloride, 0.1 M Bicine, 20% w/v Polyethylene Glycol Monomethyl Ether 550, pH 9.0, VAPOR DIFFUSION, temperature 289K |