2OGK

Crystal structure of protein AF2318 from Archaeglobus fulgidus, Pfam DUF54

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-15
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97960
Spacegroup name	P 21 21 21
Unit cell lengths	38.430, 156.300, 174.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.700 - 3.000
R-factor	0.262
Rwork	0.262
R-free	0.30900
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.120
High resolution limit [Å]	3.000	3.000
Rmerge	0.084	0.446
Number of reflections	21971
<I/σ(I)>	8.7	2.3
Completeness [%]	99.5	99.1
Redundancy	6.6	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	PEG MME 5000, HEPES Buffer, Tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7	293	PEG MME 5000, HEPES Buffer, Tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K