2OGB
Crystal structure of the C-terminal domain of mouse Nrdp1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-27 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.427, 58.227, 37.387 |
| Unit cell angles | 90.00, 101.53, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.213 |
| Rwork | 0.212 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.373 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.03) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
| Rmerge | 0.092 | 0.077 | 0.336 |
| Number of reflections | 20628 | ||
| <I/σ(I)> | 25.3 | ||
| Completeness [%] | 85.2 | 85.1 | 56.6 |
| Redundancy | 4.3 | 5.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 25% (w/v) polyethylene glycol 8000, 180 mM potassium thiocyanate, 100 mM Tris-HCl, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
