2OG9
Crystal Structure of mandelate racemase/muconate lactonizing enzyme from Polaromonas sp. JS666
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97900 |
Spacegroup name | I 4 |
Unit cell lengths | 133.416, 133.416, 87.434 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.840 - 1.900 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.23000 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.380 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 60304 | |
<I/σ(I)> | 18.3 | 3.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.1 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | VAPOR DIFFUSION, SITTING DROP |