2OG5
Crystal structure of asparagine oxygenase (AsnO)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-30 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 91.610, 91.610, 90.878 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.910 - 1.450 |
| R-factor | 0.171 |
| Rwork | 0.170 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dry |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.175 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.060 | 0.992 |
| Number of reflections | 78000 | |
| <I/σ(I)> | 12.6 | 0.9 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.85 | 3.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 2.5 M sodium acetate, 0.1 M Hepes, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
