2OG3
structure of the rna binding domain of n protein from SARS coronavirus in cubic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-23 |
| Wavelength(s) | 0.9797 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 110.001, 110.001, 110.001 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.900 - 1.850 |
| R-factor | 0.20133 |
| Rwork | 0.199 |
| R-free | 0.24711 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ofz |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.431 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.075 | 0.470 |
| Number of reflections | 17989 | |
| <I/σ(I)> | 29.33 | 1.91 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 277 | 40% MPD and 0.1 M Tris pH 8.0, typically crystals appear within 2 weeks, VAPOR DIFFUSION, temperature 277K |
