2OG2
Crystal structure of chloroplast FtsY from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 87.049, 84.209, 98.927 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.525 - 2.000 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vma |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.618 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.525 | 49.460 | 2.110 |
| High resolution limit [Å] | 2.000 | 6.330 | 2.000 |
| Rmerge | 0.077 | 0.039 | 0.577 |
| Total number of observations | 6148 | 22698 | |
| Number of reflections | 24849 | ||
| <I/σ(I)> | 6.9 | 14 | 1.3 |
| Completeness [%] | 99.7 | 99.4 | 98.5 |
| Redundancy | 7.8 | 7.1 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 298 | 1.2M sodium malonate, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
