2OG2

Crystal structure of chloroplast FtsY from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-11
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	I 2 2 2
Unit cell lengths	87.049, 84.209, 98.927
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.525 - 2.000
R-factor	0.213
Rwork	0.211
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vma
RMSD bond length	0.004
RMSD bond angle	0.618
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.525	49.460	2.110
High resolution limit [Å]	2.000	6.330	2.000
Rmerge	0.077	0.039	0.577
Total number of observations		6148	22698
Number of reflections	24849
<I/σ(I)>	6.9	14	1.3
Completeness [%]	99.7	99.4	98.5
Redundancy	7.8	7.1	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	298	1.2M sodium malonate, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 298K