2OG0
Crystal Structure of the Lambda Xis-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-29 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | H 3 |
| Unit cell lengths | 57.483, 57.483, 163.613 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.230 - 1.900 |
| R-factor | 0.194 |
| Rwork | 0.191 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rh6 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.170 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.409 | |
| Number of reflections | 15654 | |
| <I/σ(I)> | 20.6 | 3.71 |
| Completeness [%] | 97.8 | 82.5 |
| Redundancy | 10.32 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 30% Polyethylene Glycol monomethyl ether 2000, 0.2 M Ammonium Sulfate and 0.1 M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Polyethylene Glycol monomethyl ether | ||
| 2 | 1 | 1 | Ammonium Sulfate | ||
| 3 | 1 | 1 | Sodium Acetate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | Polyethylene Glycol monomethyl ether | ||
| 6 | 1 | 2 | Ammonium Sulfate | ||
| 7 | 1 | 2 | Sodium Acetate |
