2OFR
1.00 A Crystal Structure Of V36A/D129A/L130A Mutant of Nitrophorin 4 From Rhodnius Prolixus Complexed With Nitric Oxide at pH 5.6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 70.191, 42.637, 52.975 |
Unit cell angles | 90.00, 94.12, 90.00 |
Refinement procedure
Resolution | 9.470 - 1.000 |
R-factor | 0.127 |
Rwork | 0.126 |
R-free | 0.14795 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1sxx |
RMSD bond length | 0.020 |
RMSD bond angle | 1.983 |
Data scaling software | CrystalClear |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.850 | 1.040 |
High resolution limit [Å] | 1.000 | 1.000 |
Rmerge | 0.100 | 0.390 |
Number of reflections | 79668 | |
<I/σ(I)> | 37 | 5.33 |
Completeness [%] | 94.1 | 90.1 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K |