2OFK
Crystal Structure of 3-methyladenine DNA glycosylase I (TAG)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.000, 0.9793, 0.9718, 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.455, 63.681, 62.127 |
| Unit cell angles | 90.00, 106.94, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.500 |
| R-factor | 0.16283 |
| Rwork | 0.161 |
| R-free | 0.19555 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.427 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 64266 | |
| <I/σ(I)> | 23.8 | 4.8 |
| Completeness [%] | 93.6 | 61.1 |
| Redundancy | 7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 30% PEG 200, 5% PEG 3000, 100 mM MES pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
