2OFJ
Crystal structure of the E190A mutant of o-succinylbenzoate synthase from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979462 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.475, 77.388, 110.677 |
| Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
| Resolution | 43.291 - 2.300 |
| R-factor | 0.225 |
| Rwork | 0.222 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fhu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.278 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.291 | 51.780 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.131 | 0.018 | 0.018 |
| Total number of observations | 3065 | 16566 | |
| Number of reflections | 76466 | ||
| <I/σ(I)> | 4.5 | 14 | 0.3 |
| Completeness [%] | 96.5 | 93.4 | 82.8 |
| Redundancy | 3.7 | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 298 | 0.1 M MES, 75 mM Sodium molybdate, 25% (v/v) PEG 4000, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
