2OFF
The crystal structure of Glycogen Phosphorylase b in complex with a potent allosteric inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2006-10-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.780, 128.780, 116.051 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 90.900 - 2.200 |
| R-factor | 0.18525 |
| Rwork | 0.184 |
| R-free | 0.21316 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.071 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.900 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 48704 | |
| <I/σ(I)> | 11.1 | 4.7 |
| Completeness [%] | 97.2 | 95.5 |
| Redundancy | 6.3 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | Bes 10mM, EDTA, DTT, pH 6.7, SMALL TUBES, temperature 289K |
