2OFC
The crystal structure of Sclerotium rolfsii lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8441 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 99.811, 99.811, 63.991 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.490 - 1.110 |
| R-factor | 0.11963 |
| Rwork | 0.119 |
| R-free | 0.13275 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.456 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.140 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.034 | |
| Number of reflections | 127739 | |
| <I/σ(I)> | 27.4 | 13.1 |
| Completeness [%] | 98.1 | 82.3 |
| Redundancy | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.2 M ammonium acetate, 0.1 M Tris/HCl, 30% MPD, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
