2OF7
Structural Genomics, the crystal structure of a tetR-family transcriptional regulator from Streptomyces coelicolor A3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97902 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 75.101, 73.240, 48.082 |
Unit cell angles | 90.00, 107.27, 90.00 |
Refinement procedure
Resolution | 36.610 - 2.300 |
R-factor | 0.23389 |
Rwork | 0.231 |
R-free | 0.29270 |
Structure solution method | SAD |
RMSD bond length | 0.025 |
RMSD bond angle | 2.616 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.610 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.070 | 0.282 |
Number of reflections | 10963 | |
<I/σ(I)> | 28.5 | 3.3 |
Completeness [%] | 98.8 | 91.6 |
Redundancy | 4.6 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M NH4(OAC), 25% PEG3350, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |