2OEQ
Protein of Unknown Function (DUF964) from Bacillus stearothermophilus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-02 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97907, 0.97923 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.901, 54.353, 59.629 |
| Unit cell angles | 69.67, 81.93, 72.57 |
Refinement procedure
| Resolution | 34.170 - 2.900 |
| R-factor | 0.23164 |
| Rwork | 0.222 |
| R-free | 0.31437 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.576 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.900 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.106 | 0.513 |
| Number of reflections | 10827 | |
| <I/σ(I)> | 8.2 | 2.1 |
| Completeness [%] | 94.8 | 70.6 |
| Redundancy | 4.25 | 2.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 295 | 0.2M Potassium dihydrogen phosphate, 15% PEG3350, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
