2OEN
Structural mechanism for the fine-tuning of CcpA function by the small molecule effectors glucose-6-phosphate and fructose-1,6-bisphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-23 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 69.330, 69.330, 229.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.370 - 3.170 |
| R-factor | 0.278 |
| Rwork | 0.278 |
| R-free | 0.31800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nzu |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.400 | 3.370 |
| High resolution limit [Å] | 3.170 | 3.170 |
| Rmerge | 0.056 | 0.340 |
| Number of reflections | 10070 | |
| <I/σ(I)> | 12 | 1.2 |
| Completeness [%] | 98.9 | 99.1 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | ammonium sulphate, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
